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Cys 2 ) and cystine-free (Cys 2 -free) medium with vehicle (DMSO) or RSL3 (250 nM), alone or with Fer-1 (10 µM) or Trolox (100 µM)

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Patients with idiopathic dilated cardiomyopathy exhibit elevated copper but reduced serum zinc compared to healthy controls, supporting previous findings linking zinc deficiency to cardiac health [82]